BIOMMAT Team

The BioMMAT team develops complex biofunctional polymer systems in the form of (i) ultrathin coatings for the surface of biomaterials, and (ii) porous membranes and hydrogels for use as tissue regeneration supports or 3D tissue models. The issues of osseointegration of orthopedic and dental implants, bone regeneration and biomimetic modeling of healthy or pathological tissues are mainly targeted.

Keywords: Biofunctional surfaces; Nano-micro-structured surfaces; Biomimetic microenvironment; Layer-by-layer films; Cell-substrate interactions; Osteogenesis

Examples of achievements:

• Biomimetic osteogenic and antimicrobial Layer-by-Layer (LbL) coatings based on polysaccharides and polypeptides
  • Caractérisation structurale et contrôle de la nucléation hétérogène de l’hydroxyapatite (https://www.theses.fr/152668012)
  • Modulation des propriétés mécaniques (méthodes de réticulation) et contrôle de la différenciation cellulaire ostéogénique. (https://www.theses.fr/2013ROUES047)
    
    (A) Red autofluorescence of genipin, cross-linking LbL films. (B) Topography of an LbL film obtained by AFM. (C) Fluorescence of osteoblasts grown on LbL films. (D) Surface coating of titanium alloy by LbL films – mineralization of the extracellular matrix characterized by confocal Raman microspectroscopy.
  • LbL nanoreservoirs: Incorporation of osteogenic compounds vectored as inclusion complexes with cyclodextrins, and incorporation of antimicrobial peptides (BRICS team collaboration) (https://www.theses.fr/2021NORMR021)
    
    Antibacterial impact (Staphylococcus aureus) of LbL films of chondroitin sulfate and poly-L-lysine enriched in nisin Z.
• Porous membranes and hydrogels
  • 3D tissue modeling: influence of biochemical and mechanical properties of hyaluronic acid hydrogels functionalized by extracellular matrix molecules on cancer cells (SCC team collaboration). (https://tel.archives-ouvertes.fr/tel-03252427)
    
    Hyaluronic acid hydrogel functionalized by extracellular matrix molecules. (A) Porous hydrogel observed by SEM. (B) Clusters of Glioblastoma cells within the hydrogels observed by microscopy.
  • Formulation of composite hydrogels based on proteins/clays for the improvement of thermomechanical properties, and as patches for the regeneration of chronic wounds.
  • Development of immunomodulation strategies for tissue regeneration, via the polarization of macrophages in hydrogels functionalized by polyunsaturated fatty acids.